Journal: Cornea

Article Title: Comparison of galectin expression signatures in rejected and accepted murine corneal allografts

doi: 10.1097/ICO.0000000000000439

Figure Lengend Snippet: Immunofluorescence localization of galectins in normal corneas and corneas with rejected and accepted allografts. Frozen tissue sections were immunostained using antibodies against galectins-1, -3, -7, -8, and -9, and Alexa fluor 488-conjugated anti-goat (for galectin-1, green), Alexa fluor 488-conjugated anti-rat (for galectins-3 and -9, green) and Alexa fluor 568-conjugated anti-rabbit (for galectins-7 and -8, red), followed by counterstaining with DAPI (blue). White arrows indicate the interface between donor (D) and recipient (R). Immunostaining processing and exposure time of images in rejected and accepted allografts were the same. Note that in normal corneas, galectin-1 is expressed mainly in corneal stroma, galectin-3 is expressed mainly in corneal epithelium, and galectins-7, -8 and -9 are present in both epithelium and stroma. In both rejected and accepted allografts, immunoreactivity of the five lectins is detected in both corneal epithelium and stroma. No immunoreactivity was detected in control corneas which were treated the same way as the experimental group except that isotype control IgG was used as primary antibody or the step involving incubation with the primary antibody was omitted (negative control, data not shown). N=3 for each galectin. Bar = 50 μm.

Article Snippet: Specific reagents included: Odyssey® blocking buffer (OBB) from Li-Cor Biosciences (Lincoln, NE); goat anti-mouse galectin-1 from R&D Systems (Minneapolis, MN); a hybridoma secreting rat anti-human/mouse galectin-3 (mAb M3/38) from ATCC (Manassas, VA); rabbit anti-mouse galectin-7 from Bethyl Laboratories (Montgomery, TX); rabbit anti-human/mouse galectin-8 from Novus Biologicals (Littleton, CO); rat anti-mouse galectin-9 (clone 108A2) from BioLegend (San Diego, CA); anti-β-actin (clone AC-15) from Santa Cruz Biotechnology (Dallas, TX).

Techniques: Immunofluorescence, Immunostaining, Incubation, Negative Control

Journal: Oncotarget

Article Title: Galectin-7 is epigenetically-regulated tumor suppressor in gastric cancer

doi:

Figure Lengend Snippet: (A) mRNA and protein expression of galectin-7 in nine gastric cancer cell lines by RT-PCR and western blot. GAPDH and β-actin were used as loading controls. (B-E) AGS cells were transfected with pQE control vector and pQE-galectin-7 expression vector for 48 h. (B) Western blot analysis of galectin-7 protein expression and crystal violet staining of cells, (C) Cell proliferation measured by WST assay, (D) Cell migration assay, (E) Cell invasion assay. For cell migration and invasion assays the p value (* p <0.0001 vs. scRNA) was calculated using Student's t-test.

Article Snippet: The primary antibodies for galectin-7 and β-actin, and HRP-conjugated goat anti-mouse or goat anti-rabbit IgGs were purchased from Santa Cruz Biotechnology and the signals were detected using an enhanced chemiluminescence (ECL) plus kit (Amersham Life Science) and a LAS-3000 (Fujifilm) detector according to the manufacturer's directions.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Transfection, Control, Plasmid Preparation, Staining, WST Assay, Cell Migration Assay, Invasion Assay, Migration

Journal: Oncotarget

Article Title: Galectin-7 is epigenetically-regulated tumor suppressor in gastric cancer

doi:

Figure Lengend Snippet: (A-B) mRNA expression of galectin-7 in 26 cancer cell lines detected by RT-PCR (A) and RT-qPCR (B). β-actin was used as a loading control. (C) MSP analysis of the galectin-7 gene in 14 cancer cell lines. The methylated (M) and unmethylated (U) DNA was amplified using primers specific for each methylation status. Universal methylated DNA was used as a positive loading control.

Article Snippet: The primary antibodies for galectin-7 and β-actin, and HRP-conjugated goat anti-mouse or goat anti-rabbit IgGs were purchased from Santa Cruz Biotechnology and the signals were detected using an enhanced chemiluminescence (ECL) plus kit (Amersham Life Science) and a LAS-3000 (Fujifilm) detector according to the manufacturer's directions.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Control, Methylation, Amplification